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The primary in vitro evaluation of test compounds is performed against a broad panel of pathogens to allow proper evaluation of selectivity. This approach involves integrated and robotic logistics to attain a reasonably high throughput. Compound stock solutions are prepared in 100% DMSO at 20 mM or mg/ml. The compounds are serially pre-diluted (2-fold or 4-fold) in DMSO followed by a further (intermediate) dilution in demineralized water to assure a final in-test DMSO concentration of <1%. Test plates are identical for all screens and are produced as a single batch.
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