Leishmaniasis Two Leishmania species (L.infantum MHOM/MA(BE)/67 and L.donovani MHOM/ET/67/L82) are used. The strains are maintained in the Golden Hamster and spleen amastigotes are collected for preparing infection inocula. Primary peritoneal mouse macrophages are used as host cell and are collected 2 days after peritoneal stimulation with a 2% potato starch suspension. Assays are performed in 96-well microtiter plates, each well containing 10 µl of the compound dilutions together with 190 µl of macrophage/parasite inoculum (3.105 cells + 3.106 parasites/well // RPMI-1640 + 5% FCSi). After 5 days incubation, parasite burdens (mean number of amastigotes/macrophage) are microscopically assessed after Giemsa staining. The results are expressed as % reduction in parasite burden compared to untreated control wells and an IC50 (50% inhibitory concentration) is calculated. Pentostam® and miltefosin are included as the reference drug.