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The Trypanosoma brucei brucei Squib 427 strain (suramin-sensitive) is used. The strain is maintained in Hirumi (HMI-9) medium, supplemented with 10% FCSi. Assays are performed in 96-well microtiter plates, each well containing 10 µl of the compound dilutions together with 190 µl of the parasite suspension (7.104 parasites/ml). After 3 days incubation, parasite growth is assessed fluorimetrically after addition of resazurin. After 24 hours at 37°C, fluorescence is measured (lex 550 nm, lem 590 nm). The results are expressed as % reduction in parasite growth/viability compared to control wells and an IC50 (50% inhibitory concentration) is calculated. Suramin is included as the reference drug.
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