|Course code module||2BBIC-01|
|Study load (hours)||112|
|Instructor(s)||Xaveer Van Ostade|
|Language of instruction:||Dutch|
|Semester exam information:||semester exam in June|
|Contract restriction information:|
Basic knowledge of chemistry
Basic knowledge of biology
Structure of biopolymers
Basic knowledge of enzymology
Students wishing to include this course in their Individualised Learning Path should have acquired credits for the following courses (or these courses should be part of a study programme for which the student has passed):
Chemistry I (1BBIC-08)
Chemistry II (1BBIC-09)
Organic Chemistry (1BCHE-05)
In addition, the following courses should also be included in the Individualised Learning Path:
Physics II (2BBIC-111)
2. Objectives (expected learning outcomes)
Ability to draw up purification schemes for bioactive molecules.
Quantification and identification of bioactive molecules.
3. Course content
The course discusses the spectrophotometric and colorimetric methods for qualitative and quantitative analysis of the building blocks of biopolymers and the protein, nucleic acid, polysaccharide and lipid content in biochemical preparations.
After a discussion of the cell cultivation, cell count and division of cell types, the course focuses on methods for homogenising and fractionating cells and tissues. The methods applied for the purification and characterisation of cell organelles and their components (proteins, nucleic acids … ) are discussed at length. Separation through precipitation, filtration, centrifugation (boundary, zonal and equilibrium density centrifugation), chromatography (ion exchange, gel filtration, hydrophobic and affinity chromatography) and gel electrophoresis (agarose, polyacrylamide) are dealt with extensively.
4. Teaching method
Direct contact: Lectures
5. Assessment method
Exam: Oral, with written preparation
6. Compulsory reading – study material
7. Recommended reading - study material
- D.J. Holme, H. Peck, Analytical Biochemistry
- K. Wilson, J. Walker, Principles and techniques of Practical Biochemistry.
- N.C. Price, Proteins-Labfax.