Recombinant protein technology Course Code : 2001WETRET Study domain: Biology Bi-anuall course: Taught in academic years starting in an odd year Semester: Semester: 2nd semester Contact hours: 30 Credits: 4 Study load (hours): 112 Contract restrictions: Exam contract not possible Language of instruction : Dutch Exam period: exam in the 2nd semester Tutor(s) Yves Guisez   1. Prerequisites At the start of this course the student should have acquired the following competences: An active knowlegde of : Dutch A passive knowledge of : English General knowledge of the use of a PC and the Internet Specific prerequisites for this course: Good basic knowledge of Molecular Biology, Protein Biochemistry and Gene Technology is indispensable. 2. Learning outcomes With this course it is aimed that students gain insight into different important techniques. These techniques are nowadays at the disposal of any scientist in fundamental studies as well as in applied research, in a academic context and in the industry. Recombinant protein technology is important to everybody who wants to have small or important quantities of high quality pure recombinant protein. 3. Course contents This course is in fact an advanced study of molecular biotechnology. Attention is given to prokaryotes (e.g.. Escherichia coli , Bacillus, enz…) as well as to eukaryotes (Saccharomyces cerevisiae, insect cells, cos-cells, CHO, etc…). These organisms are used as production  systems for all kind of important recombinant proteins (on lab scale as well as in bigger projects). The many examples that are treated are related to medically important proteins such as e.g. cytokines, vaccines, antibodies, etc…). A lot of attention is given to ‘downstream processing’ and protein chromatography. The most important aim is to obtain high quality end products (e.g. LPS-free). Also several tests to evaluate the quality of the products are described (bioassays, protein assays, …). 4. Teaching method Class contact teaching: Lectures 5. Assessment method and criteria Examination: Written with oral presentation Closed book Open-question 6. Study material Required reading -         Syllabus in English -         Overhead slides Optional reading The following study material can be studied on a voluntary basis: - A list with recommended reading is added to the syllabus  7. Contact information Prof. Y. Guisez Ph.D. Department of Biology University of Antwerp Campus CGB Building U 6the floor (U614) 171 Groenenborgerlaan Be-2020 Antwerpen yves.guisez@ua.ac.be Secretariat : Ms. Suzanne Pooters tel  0032 3 265 3421  (+)last update: 02/09/2011 11:20 els.moons

Inhoudsverantwoordelijke(n) : Facultaire administratie