|Course code module||MCHE20251|
|Study load (hours)||84|
|Semester exam information:||exam in the 2nd semester|
|Contract restriction information:|
Student should know the most important biomolecules (amino acids,
> nucleotides, sugars), have a basic knowledge of biochemistry,
> understand the principle of analytical techniques (such as NMR, MS)
2. Objectives (expected learning outcomes)
Understand how structure and function of biomolecules, particularly biopolymers such as polypeptides and oligonucleotides, links with their function.
Analyse conformational space of biopolymers and implications of secondary/tertiary/quaternary structure for function.
Understand how bioanalytical methods are used to investigate these structural aspects.
Applications of modern hyphenated techniques to the analysis of complex samples from biological systems.
3. Course content
Biochemical building blocks (amino acids, nucleotides, sugars).
Biopolymers and their conformational analysis.
Preferred structural arrangements of biopolymers and their characteristics.
Noncovalent interactions between biomolecules in the cellular context.
Basic bioanalytical and biophysical methods: NMR, MS, IR, UV/CD spectroscopy, ITC, SPR etc.
Case studies of successful application of bioanalytical and biophysical methods in Molecular Biology.
4. Teaching method
Direct contact: LecturesSeminars (possible question and answer sessions)
Personal work: Assignments - individual
5. Assessment method
Exam: Written, without oral presentationOpen questions
6. Compulsory reading – study material
reader with selected literature, will be defined at course start
7. Recommended reading - study material
Selected review papers
The teacher is available for further questions