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Frank Sobott  
    

e-mail:
    Frank.Sobott@ua.ac.be
telefoon:
    032653388
fax:
    032653233
statuut:
    Zelfst. acad. pers. BOF
functie:
    hoofddocent
afdeling:
    Departement Chemie
interne mandaten:
    
bedrijfsadres:
    Middelheimcampus
G.V.408
Groenenborgerlaan 171
2020 Antwerpen

 

Prof. Dr. Frank Sobott

2000        Ph.D., University of Frankfurt/Main, Germany (Institute for Physical and Theoretical Chemistry).

                "Characterization and application of LILBID - laser desorption mass spectrometry", supervised by Prof. Dr Bernhard Brutschy and Prof. Dr. Michael Karas.

2000         Visiting Scientist (National Institute for Advanced Interdisciplinary Research), Tsukuba, Japan.

2000-04   Post-doctoral Research Associate, Prof. Carol V. Robinson (Chemistry Department), University of Oxford and University of Cambridge, U.K.

2004-09   Team leader - Molecular Biophysics (Structural Genomics Consortium); Senior scientist - Oxford Centre for Integrative Systems Biology; Core scientist - Proteomics,
                (Oxford Centre for Gene Function/OXION), Principal Investigator - Laboratory of Molecular Biophysics (Department of Biochemistry), University of Oxford, U.K.

2009-       Visiting Scientist (Department of Biochemistry), University of Oxford, U.K.


Research themes
Research themes.jpg

PhD students and postdocs: Enquiries with C.V. welcome.

Key publications

1) F. Sobott, W. Kleinekofort, B. Brutschy. Cation selectivity of natural and synthetic ionophores probed with laser induced liquid beam mass spectrometry.

Anal. Chem. 69 (1997), 3587.

2) F. Sobott, S.A. Schunk, F. Schüth, B. Brutschy. Examination of condensation products of group 4 alkoxides with laser-induced liquid beam ionization/desorption mass spectrometry.

Chem. Eur. J. 4 (1998), 2353.

3) P. Bussian, F. Sobott, B. Brutschy, W. Schrader, F. Schüth. Speciation in solution: Silicate oligomers in aqueous solutions detected by mass spectrometry.

Angew. Chem. Int. Ed. 39 (2000), 3901.

4) F. Sobott, H. Hernández, M.G. McCammon, M.A. Tito, C.V. Robinson.A tandem mass spectrometer for improved transmission and analysis of large macromolecular assemblies.

Anal. Chem. 74 (2002), 1402 .

5) F. Sobott, J.L.P. Benesch, E. Vierling, C.V. Robinson.Subunit exchange of multimeric protein complexes. Real-time monitoring of subunit exchange between small heat shock proteins using electrospray-mass spectrometry.

J. Biol. Chem. 277 (2002), 38921.  

6) L.L. Ilag, H. Videler, A.R.C. McKay, F. Sobott, P. Fucini, K. Nierhaus, C.V. Robinson. Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria.

Proc. Natl. Acad. Sci. USA 102 (2005), 8192.  

7) F. Sobott, M.G. McCammon, H. Hernández, C.V. Robinson.The flight of macromolecular complexes in a mass spectrometer.

Phil. Trans. R. Soc. A 363 (2005), 379.  

8) S.H. McLaughlin, F. Sobott, Z. Yao, W. Zhang, P.R. Nielsen, G.J. Grossmann, E.D. Laue, C.V. Robinson, S.E. Jackson. The co-chaperone p23 arrests the Hsp90 ATPase cycle to trap client proteins .

J. Mol. Biol. 536 (2006), 746. 

9) F. Sobott, C.V. Robinson. Understanding protein interactions and their representation in the gas phase of the mass spectrometer.

Chapter in: Principles of mass spectrometry applied to biomolecules (Eds.: C. Lifshitz, J. Laskin), Wiley, 2006.   

10) X. Yang, W.H. Lee, F. Sobott, E. Papagrigoriou, C.V. Robinson, J.G. Grossmann, M. Sundström, D.A. Doyle, J.M. Elkins. Structural basis for protein–protein interactions in the 14-3-3 protein family.

Proc. Natl. Acad. Sci. USA 103 (2006), 17237.

11) G. Bunkoczi, E. Salah, P. Filippakopoulos, O. Fedorov, S. Müller, F. Sobott, S.A. Parker, H. Zhang, W. Min, B.E. Turk, S. Knapp. Structural and functional characterization of the human protein kinase ASK1.

Structure 15 (2007), 1215.

12) F. Sobott, S.J. Watt, J. Smith, M.J. Edelmann, H.B. Kramer, B.M. Kessler.Comparison of CID versus ETD based MS/MS fragmentation for the analysis of protein ubiquitination.

J. Am. Soc. Mass Spectrom. 20 (2009), 1652.

13)   D.J. Harvey, F. Sobott, M. Crispin, A. Wrobel, C. Bonomelli, S. Vasiljevic, C.N. Scanlan, C. Scarff, K. Thalassinos, J.H. Scrivens. Ion mobility mass spectrometry for extracting spectra of N-glycans directly from incubation mixtures following glycan release: application to glycans from engineered glycoforms of intact, folded HIV gp120.

J. Am. Soc. Mass Spectrom. 22 (2011), 568.

14) S. Brocca, L. Testa, F. Sobott, M. Šamalikova, A. Natalello, E. Papaleo, M. Lotti, L. De Gioia, S.M. Doglia, L. Alberghina, R. Grandori. Compaction properties of an intrinsically disordered protein: Sic1 and its kinase-inhibitor domain.

Biophys. J. 100 (2011), 2243.

15) F. Sobott. Characterisation of proteins and protein complexes by ion mobility-mass spectrometry.

Chemistry Today 29 (2011), 24.



 
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