- - lightmicroscopic research (conventional, fluorescence, confocal) of cells and tissues
- live cell imaging: calcium measurements, FRET and FRAP techniques
- electrophysiological intracellular recordings
- neuronal tracings
- transmission and scanning electron microscopy of cells and tissues
- (immuno)cytochemical and (immuno)histochemical research
- in situ hybridisation; RT-PCR, Western/Northern blotting
- cell and tissue cultures
- lightmicroscopical microtomy: paraffin-, cryostat- and vibratome sections
- electronmicroscopical microtomy: ultramicrotomy of tissues, cryo-ultramicrotomyTechnique: - Confocal microscopy
- Live cell imaging
- Techniques for conventional lightmicroscopy and fluorescence microscopy
- (Immuno)cytochemal and (immuno)histochemical techniques
- Cell and tissue cultures
- Vibratome
- Intracellular injections and recordings
- (Cryo)ultramicrotomy, transmission- and scanning electronmicroscopy
- Stereology (Olympus/Analysis)
- 3D analysis
- RT-PCR
Users: - Clinical biology
- Pharmaceutical industry
- Hospitals
- Research institutes
Keywords: IMMUNOCYTOCHEMISTRY, CONFOCAL MICROSCOPY, LIVE CELL IMAGING, FLUORESCENCE MICROSCOPY, CELL CULTURES, TRANSMISSION ELECTRON MICROSCOPY, SCANNING ELECTRON MICROSCOPY - The interfaculty research group 'Cell Biology and Histology' has the responsibility to enlarge the core facility 'Biomedical microscopic imaging' and to support the community of the university concerning microscopic imaging.
Available services/expertise:
- scientific and technical support in setting up new experiments belonging to the expertise of the core facility, and aid with interpretation of the results
- technical and scientific support of researchers and students at the different microscopy systems of the core facility, including image recording, -processing and -analysingTechnique: - Infrastructure, materials, knowledge and skills regarding different microscopy systems [light-, fluorescence-, confocal - and electron microscopy (scanning and transmission),...] and their biomedical microscopic applications
- Various techniques regarding embedding, cutting and staining of light-microscopic preparation (cryostat and paraffine sections)
- Support and/or carry out complex immunocytochemical multilabelling procedures, including visualisation and interpretation of results
- Support and/or carry out in situ hybridisation and (qualitative) RT-PCR protocols
- Various techniques for embedding, ultramicrotomy , and contrast enhancement of transmission electron microscopic preparations of various biological specimens.
- Preparation of TEM samples by high-pressure freezing and freeze substitution
- Cutting vibratome slices and further processing for live cell experiments (confocal live cell imaging, electrophysiology,...)
- Laser capture microdissection of cell(group)s for different applications
Users: Collaborators of the research group Cell Biology and Histology support different external researchers (UA-related and others) in view of the UA core facility BMB and regarding scientific collaborations. Support is given for microscopy systems and the accompanying preparation techniques (embedding, microtomy, staining,...).
Keywords: HISTOLOGY, CONFOCAL MICROSCOPY, LIVE CELL IMAGING, ELECTRON MICROSCOPY, MOLECULAR DIAGNOSTICS, PCR ANALYSIS
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